The Association for Biology Laboratory Education

June 1 - 5, 1999

The University of Nebraska-Lincoln
Lincoln, Nebraska

The 21st annual conference of the Association of Biology Laboratory Education (ABLE) will be held in Lincoln, Nebraska, June 1-5, 1999, at the University of Nebraska-Lincoln (UNL). The conference is being sponsored by the School of Biological Sciences and the College of Arts and Sciences as part of an initiative to promote improvement of Math and Science Education.


Conference Highlights

Monday, May 31

  • ABLE Board Meeting: 3:30 pm - 5:00 pm; 6:30 pm - 9:30 pm
  • Conference Registration: 7:00 pm - 9:00 pm - Pound Residence Hall

Tuesday, June 1

  • Conference Registration: 7:30 am - 10:00 am - Pound Residence Hall
  • Field Trip 1: Agricultural Research and Development Center (8:15 am - 5:00 pm)
  • Field Trip 2: Exploring the Platte River (8:00 am - 5:00 pm)
  • Field Trip 3: Planes, Fish, and Wildlife (8:30 am - 5:00 pm)
  • Conference Registration: 3:00 pm - 5:30 pm - Pound Residence Hall
  • Welcome Reception: UN State Museum (6:30 pm - 8:30 pm)

Wednesday, June 2

  • Conference Registration:7:00 am - 8:00 am - Pound Residence Hall
  • Major Workshops: 8:00 am - 11:00 am
  • Special Lecture and Box Lunch : 11:30 am - 12:30 pm
  • Mini-Workshops: 12:45 pm - 1:45 pm
  • Major Workshops: 2:15 pm - 5:15 pm
  • Special Event: Pow Wow - 6:00 pm - 9:00 pm

Thursday, June 3

  • Major Workshops: 8:00 am - 11:00 am
  • Conference Photograph and Box Lunch 11:15 am - 12:15 pm
  • Mini Workshops: 12:15 pm - 2:15 pm
  • Major Workshops: 2:30 pm - 5:30 pm
  • ABLE Business Meeting 5:45 pm - 7:00 pm

Friday, June 4

Saturday, June 5

  • Field Trip 4: AshFall Fossil Beds (7:45 am - 9:30 pm)
  • Field Trip 5: Konza Prairie (8:00 am - 9:00 pm)
  • Field Trip 6: Indian Cave State Park/Squaw Creek Wildlife Refuge (8:15 am - 9:00 pm)


Conference Information Contacts

Registration Information:

Tom Graf, Program Coordinator
Academic Conferences and Professional Programs
University of Nebraska-Lincoln, Room 156 NCCE
33rd & Holdredge Streets, Lincoln, NE 68583-9600
Phone (402) 472-2844, Fax: (402)472-9688
Email: tgraf@unlinfo.unl.edu

Conference Program Information

Dr. Bill Glider, ABLE Conference Host
School of Biological Sciences
University of Nebraska-Lincoln, Lincoln, NE 68588-0118.
Phone: (402) 472-6242, Fax: (402) 472-2083
Email: bglider@unlinfo.unl.edu

(Emergency phone numbers for use during the conference will be listed in the registration confirmation letter.)


The City of Lincoln

Lincoln, Nebraska's capital city, is located in the southeastern part of the state. In the 1850s, the salt flats west of the city attracted the first settlers to what would become Lancaster County. It was hoped mining of the valuable salt deposits would create major industry in the area. By 1867, Nebraska had become a state and the village of Lancaster, renamed Lincoln in honor of the U.S. president, had been designated as its capital. The village grew quickly with the chartering of the University in 1869 and the arrival of the Burlington & Missouri Railroad in 1870. Now with a population of more than 200,000, Lincoln has become the seat of the only one-house legislature (the Unicameral) in the nation. The city provides a wide variety of urban arts and entertainment and prides itself on a quality lifestyle that includes safe streets and a clean environment. The downtown retailing area and central business district is within easy walking distance of the University's City Campus. For more information about Lincoln and the many offerings located in the "Star City", please visit the Lincoln Convention and Visitors Bureau web site.



University of Nebraska-Lincoln

The University of Nebraska-Lincoln (UNL) was chartered by the Legislature in 1869 as the state's public university and land grant institution It is the largest of four campuses with an enrollment of about 24,000 undergraduate and graduate students. Instruction at UNL is organized within individual colleges and schools located on two campuses, City and East. The Colleges of Arts and Sciences, Engineering, Teachers, Fine Arts, and Business Administration are located on City Campus and the College of Agricultural Sciences and Natural Resources is located on East Campus, about 3 miles from City Campus. The School of Biological Sciences, within the College of Arts and Sciences, is divided into two Sections: Ecology, Evolution & Behavioral Biology and Genetics, Cellular & Molecular Biology. The main facilities of the School are located in Manter Hall of Life Sciences and the Beadle Center for Genetics and Biomaterials Research.



Weather

The weather in Lincoln is anything but predictable in early June. It may be cold and rainy or hot and humid. Daytime temperatures average around 76 oF (24 oC) and nighttime temperatures 52 oF (11 oC). In addition, June is often one of the wettest months in southeast Nebraska. Therefore, we recommend that you pack an umbrella or light, waterproof rain jacket.


Transportation

Airlines

The Lincoln Municipal Airport is served by United (via Denver and Chicago), TWA (via St. Louis), Northwest (via Minneapolis) and USAir (via Kansas City). Participants may wish to consider flying into Omaha's Eppley Airfield (50 miles east of Lincoln) which is served by a greater variety of airlines. For information regarding Omaha's Eppley Airfield, call (402) 422-6817 or visit their web site. United Airlines is the official carrier for the ABLE Conference and is offering a discount rate for travel. For information call AAA Business Travel at (800) 456-3002 or any authorized United Airlines ticket vendor. Be sure to indicate that you are attending the ABLE Conference (code: 527MH) in order to obtain the discounted fare. The taxi fare from the Lincoln Municipal Airport to downtown Lincoln is about $15.

Ground Travel

Car rentals from most major companies are available at both Lincoln Municipal Airport and Eppley Airfield. Advanced booking is advised. Additionally, shuttle service between the Omaha and Lincoln airports is provided by Eppley Express. Call (800) 888-9793 for details. Note that the Eppley Express does not make stops in downtown Lincoln or at UNL. Taxicab service is available through Husker Cabs at (402) 477-4111and StarTran, Lincoln's public transportation system, operates buses between the hours of 7:00 a.m.- 6:00 p.m., Mondays-Saturdays. For more information about StarTran schedules, call (402) 476-1234.

Parking

ABLE participants staying in the Pound Residence Hall will be issued a permit to park their vehicle in a nearby lot when they check in. ABLE participants who are staying off campus have several options for parking. The Stadium Drive Parking Garage is located at 625 Stadium Drive (on the west side of Memorial Stadium) and is less than a five minute walk from Manter Hall, home to the School of Biological Sciences. Rates for this facility are reasonably priced. Additionally, visitors can secure parking permits from UNL Parking Services, located in the Stadium Drive Parking Garage.



Accommodations

Conference Housing

Participants electing to stay on campus during the ABLE conference will be housed in Pound Residence Hall, located at 513 North 17th. Pound Hall is within walking distance of all conference sites. Each air-conditioned room features two single beds and all linens are provided. The rooms are served by communal restroom facilities. A single-occupancy room costs $31.00 per day and a double occupancy room costs $17.50 per day. These rates include a full breakfast following each night's stay. Registration forms for on-campus housing are enclosed in this booklet.

Additional Lodging

A block of rooms has been reserved for ABLE conference participants at the Holiday Inn Downtown located at 141 North 9th Street. The Holiday Inn is several blocks southwest of the UNL City Campus and within walking distance of conference activities. Rooms include either a king sized bed or two double size beds. The Holiday Inn features an indoor pool, restaurant, and lounge. Rooms can be secured for $79.00 if reserved by Monday, May 3, 1999 (does not include 9% room tax). Call (402) 475-4011 or (800) 432-0002 for reservations. A number of budget motels are located near the Lincoln Municipal Airport, which is about 10 miles from campus. Information concerning these motels can be obtained from the City of Lincoln web site.

Camping

There are a number of campgrounds within driving distance from the UNL campus. A description of these facilities can be found in the Visitor Information/Lodging section on the Lincoln web site.



Food Service

Breakfast: Conference attendees who stay at the Pound Residence Hall will be provided full breakfast through their dormitory accommodation.

Lunch: Box lunches will be provided during the conference and on field trips as a part of the registration fee.

Dinner: Dinners are not provided through conference registration, although several special event dinners can be paid for along with the registration fees (see section on Special Events).

A listing of recommended restaurants in Lincoln will be included with the conference packet at check-in.



Campus Recreation Facility

During the conference, ABLE participants who wish to maintain their regular exercise routines while away from home will have access to Campus Recreation exercise facilities. Located one half block north of 14th & Vine St., the Sapp Recreation facility features both an indoor and outdoor track, a swimming pool, weight room, basketball and raquetball courts. The Sapp Recreation Center is open from 6:30 am - 10:00 pm daily. Participants may sign up for one week access for $6.00 by presenting their residence hall meal tags.



Registration

Conference and housing registration forms...

Payment must accompany each registration form in order for it to be processed. Please submit separate conference and housing registration forms for each participant. Blank registration forms may be photocopied. Payment must be made by check or money order to the University of Nebraska in U.S. funds or by VISA, MasterCard, or American Express credit card. Early registration (deadline May 3) costs $180. This registration fee includes the Welcome Reception, a box lunch (June 1-June 5), workshops, workshop documents, and refreshments.

The conference is limited to 150 participants. Space will be assigned in the order that registration forms are received; priority will be given to ABLE members whose dues are paid by May 3. Registration fee for attendees who are not members of ABLE is $200. There is an additional $25 late registration fee for both members and nonmembers after May 3. Accompanying persons may register for $45. and are invited to attend the Welcome Reception, lunches, and refreshment breaks.

Pre- and post-conference field trips, the Conference Dinner, and the Pow Wow are not included with the registration fee and must be purchased before the conference.

Inquiries regarding conference registration should be directed to:

Tom Graf, Program Coordinator, Academic Conferences and Professional Programs, University of Nebraska-Lincoln, Room 156 NCCE, 33rd & Holdredge Streets, Lincoln, NE 68583-9600. Phone (402) 472-2844, Fax: (402)472-9688, Email: tgraf@unlinfo.unl.edu

Inquiries regarding the conference program should be directed to:

Dr. Bill Glider, ABLE Conference Host, School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0118. Phone: (402) 472-6242, Fax: (402) 472-2083, Email: bglider@unlinfo.unl.edu

Refund Policy

If conference registration must be canceled, fees already paid will be refunded upon receipt of a written request (fax is acceptable) to the Department of Academic Conferences and Professional Programs on or before May 3. No refunds will be issued after May 3.



ABLE Membership

Registration priority will be given to persons who are current members of ABLE as of May 3, 1999. The Membership fee is $35 annually and includes the Conference Proceedings and the newsletter Labstracts.

You may join ABLE or renew your membership as part of your registration fees or by contacting:

Nancy Rosenbaum
ABLE Membership Chairperson
Biology Department, Box 208104, Yale University, New Haven, CT 06520-9104
Phone: (203) 432-3864
Fax: (203) 432-3854
E-mail: nancy.rosenbaum@yale.edu

T-Shirts

The official ABLE 1999 conference t-shirt will feature the conference logo as displayed at the top of this document. The logo is an original drawing of a Burrowing Owl by Dr. Paul Johnsgard, a world renowned ornithologist and Regents Professor of Biological Sciences at UNL. Burrowing Owls are commonly found nesting in old prairie dog burrows in Nebraska grasslands. T-shirts cost $12.00, paid in advance with your registration.



Laboratory Workshops

** Please register for workshop choices by both day and number.

Wednesday, June 2
All workshops presented 8:00 am - 11:00 am and 2:15 pm - 5:15 pm unless noted otherwise

1. Case It! Case Study Learning
Mark Bergland and Karen Klyczek (University of Wisconsin - River Falls)

Case It! is an NSF-sponsored BioQUEST project to promote collaborative case-based learning in biology education worldwide. Students use open-ended molecular biology computer simulations to analyze case studies in a variety of fields including human genetics, forensics, and ecology, then discuss results with their peers at other institutions via web-based "poster sessions". Workshop participants will use software modules to gather background information and analyze DNA sequences, then create web-page posters and discuss them via a conferencing system at the Case It! web site. Integration of the simulations into wet labs involving DNA digestion and PCR amplification will also be discussed.

2. Seafood Forensics
Kathy Frame (National Association of Biology Teachers)

Students perform protein analysis of crab tissue to determine if species substitution has occurred. Samples are analyzed using horizontal agarose gel electrophoresis. This hands-on, investigative, "cutting-edge" activity is one of 17 in the publication Shoestring Biotechnology. The publication emphasizes biotechnology's role in the environment, medicine, plant and animal preservation, and agriculture, as well as how to teach biotech cost-effectively (NSF/ATE/DUE #9553720).

3. Paleoecology as a Classroom Tool to Address Global Climate Change
Mark E. Lyford and Jane M. Beiswenger (University of Wyoming)

We have designed an activity to illustrate paleoecological techniques with a focus on the Pleistocene period. We provide students with background information and ask them to count pollen on slides representing six different time periods from a site in Idaho. Based on the class data, we ask the students to interpret how the vegetation has changed at the site. The students then infer how the climate would have changed given the ecological characteristics of the species examined. We conclude with a discussion of how interpreting past vegetation changes may help predict future vegetation patterns in response to climate change.

4. Electromyography: Recording Electrical Signals from Human Muscle
Charlie Drewes (Iowa State University)

You will use surface recording electrodes, a small battery-powered pre-amplifier, and a small battery-powered audio-monitor (for purposes of biofeedback) to analyze electromyogram (EMG) activity from the gastrocnemius muscle in a freely moving, untethered human. Also, you will (1) learn principles of EMG recording, (2) study electrical activity patterns from single motor units, (3) examine patterns of motor unit recruitment during voluntary movement, (4) study the graded nature of involuntary stretch reflex responses, (5) measure stretch reflex latency with an oscilloscope, and (6) calculate average sensori-motor conduction velocity in human leg nerve.

5. Population Genetics and Evolution
Christine K. Barton (Centre College)

The Hardy-Weinberg law represents the cornerstone of the modern theory of evolution. In an infinitely large population with completely random mating, this law establishes a mathematical model that allows us to accurately predict both gene and genotype frequencies. Biologists use the predictions of the Hardy-Weinberg law as a standard against which microevolutionary change can be measured. In this lab exercise, students are presented with a hands-on simulation that allows them to become acquainted with an equilibrium population and the subsequent effect that gene flow, natural selection, and genetic drift could have on the original gene pool. The hands-on simulation is supplemented with a computer exercise using the EVOLVE software program.

6. Plant Vascular Systems
Laura K. Thompson (Furman University)

One of the most important developments shown by plants which make them suited to life on dry land is a vascular system which distributes water, nutrients and sugars throughout the plant from their disparate sources (leaves, roots, atmosphere, soil, etc.). This freshman level laboratory exercise introduces students to plant diversity by using an investigative approach in studying various aspects of the plant vascular system. Students study the anatomy of the xylem system in a dicot stem, measure the xylem flow rate, and study the effect of microenvironmental conditions that control stomatal opening.

7. An Evaluation of Host-Parasite Interaction
Sara V. Brant and Ben Hanelt (University of Nebraska-Lincoln)

Parasitism is the prevailing life-style and host-parasite associations provide outstanding models in studies of species interactions. This exercise demonstrates some basic principles in parasitology using a simple and easily obtainable systems such as rat/tapeworms, frogs/various parasites or beetles/protozoans. The following will be presented: (1) an introduction to parasite relationships with its host (2) an overview to the methods used in experimental studies (3) extrapolation of impacts on the host and other individual parasites from quantitative measures. Through experimentation, the students will understand what is a parasite, details of a complex life cycle, and how parasites interact with their environment.

8. Effective Methods of Training Biology Laboratory Teaching Assistants
Maggie Haag (University of Alberta), Kimberley Christopher (University of Alberta), Jean Dickey (Clemson University) and William V. Glider (University of Nebraska-Lincoln)

One of the most important factors in the success of undergraduate biology laboratories rests with the instructors that teach them. Often, especially in large multi-section courses, this responsibility is left in the hands of graduate or undergraduate teaching assistants (TAs). In recent years, many universities and colleges have begun to put an effort into TA training, focusing on effective and often varied means of instruction, clear and concise methods of communication, and consistent and fair marking. This workshop will present a number of examples of programs used for the training of TAs teaching biology as well as some methodologies that have proven successful. Most important to the success of this workshop will be a forum for the sharing of ideas, methods, programs, etc. that various participants have experienced. Participants are encouraged to bring appropriate TA training materials to the workshop for discussion and distribution This workshop will only be presented 2:15 - 5:15 pm

Thursday, June 3
All workshops presented 8:00 - 11:00 am and 2:30 - 5:30 pm

9. Creating Interactive Biology Tutorials for the World Wide Web Using a Simple Java Editor
William Udell (University of Nebraska-Lincoln)

This hands-on workshop will provide participants the opportunity to work with Jamba®, an easy to use and inexpensive Java editor from Interleaf Software. This program can be used to create interactive tutorials for the internet including: drag and drop, animation, fill in the blank and multiple-choice questions, and interactive slide shows. Workshop participants will first learn how to construct a basic drag and drop concept map and then how to create an interactive animation dealing with water balance in living cells. This tutorial uses a mastery based learning strategy in which the student is allowed to move on to the next question only after having successfully answered the previous question. The Jamba® software is currently available for Windows 95 and Windows NT platforms only. However, tutorials created with this software will run on Windows 95, Windows NT, Unix, and Macintosh platforms. Participants in this workshop should have experience with PC computers, and some knowledge of the web. Knowledge of HTML and Java programing is not necessary. Information about Jamba® can be found on the Jamba® web site.

10. Introducing Students to Conservation Genetics Using Sturgeon Caviar
Kathleen Nolan (St. Francis College/NY), Phaedra Doukakis, Vadim Birstein, and Rob DeSalle (American Museum of Natural History)

In this laboratory exercise students will learn how to (a) Isolate DNA from individual sturgeon eggs (available at any local deli that sells caviar) using a rapid DNAzol protocol, (b) Set up a PCR reaction using primers that have been developed for DNA from sturgeon species and (c) employ electrophoresis and methylene blue and/or ethidium bromide staining to visualize the PCR products. This laboratory exercise would allow students to contribute to a growing DNA data base on endangered species.

11. Induction of Nitrate Reductase in Plant Shoots
Barry McCashin (University of Alberta)

The induction of enzyme activity by a substrate is usually examined in prokaryotes but in this lab, we will investigate the phenomenon in a higher plant. Using a simple colorimetric assay, we will study the induction of nitrate reductase in the shoots of corn seedlings after applying a solution containing nitrate to the roots. We will look at the effect of nitrate concentration as well as the time course of enzyme induction and may also assess the role of light in the process.

12. Effects of Irradiance Level on photosynthetic CO2 Uptake and Chlorophyll Fluorescence
Stephen Hunt (Qubit Systems Inc.)

This investigation introduces students to the concept of measuring photosynthesis by open flow gas analysis, using a CO2 analyzer to measure CO2 uptake by a leaf in a leaf chamber. At the same time chlorophyll fluorescence is also measured. The photon flux supplied to the leaf is altered and effects on photosynthetic rate and chlorophyll fluorescence are measured. A photosynthetic light response curve is generated and from this students can identify the light saturation point and the light compensation point of the leaf, as well as estimating photochemical efficiency and dark respiration rate. On a separate plot, the antiparallel relationship between photosynthetic rate and chlorophyll fluorescence is shown. This investigation provides background for numerous other studies into the effects of environmental perturbations on photosynthetic physiology.

13. Investigating an Immune Response to Bacterial Infection
Jon S. Miller and David W. Stanley (University of Nebraska-Lincoln)

The ability of organisms to defend themselves from parasites and pathogens is a fundamental aspect of biology. Because of the risk of infection from blood born pathogens, and the elaborate protocols required to handle and keep mammals and other vertebrate organisms, it is becoming more difficult and expensive to conduct laboratory exercises that illustrate fundamental immune reactions. As a result, alternative models are needed to teach this important biological process. In this workshop, we suggest invertebrates, namely insects, as a solution to this growing dilemma and present activities that are appropriate for biology teaching laboratories. In this workshop you will become familiar with the internal structure of the larval tobacco hornworm, Manduca sexta and assess the nodulation response of the larvae to an artificially induced bacterial infection.

14. An Experimental System to Study Phagocytosis
Donna M. Bozzone and Denise Martin (St. Michael's College)

In this workshop, we will present a simple technique to observe and quantitate phagocytosis in the protozoan, Tetrahymena. The procedure consists of feeding ink or other inert materials to cells, and counting the number of food vacuoles that form over a 30-minute time period. Because the protocol is straightforward and the cell behavior is interesting to observe, this system is excellent both for introducing students to the microscope and for inquiry-based laboratory exploration. Workshop participants will have the opportunity to design and implement experiments that examine factors affecting phagocytosis and vacuole formation. Possible questions to explore experimentally include: will the previous nutritional state of the cells influence the rate of phagocytosis?; how will starved cells behave?; is the cytoskeleton necessary for phagocytosis?; will cells choose amongst different food items?; is the response temperature dependent? After experiments have been completed, we will discuss all results and formulate another set of testable questions.

15. Insect Predation, Prey Defense, and Community Structure
William Wyatt Hoback and Leon G. Higley (University of Nebraska-Lincoln)

Insect predators use one of three general foraging strategies to capture prey. Evolutionarily, species subjected to strong predation pressure may respond by developing defenses including poison, or by producing more offspring. This exercise examines the interplay between three potential prey species and three insect predator types played by the students by simulations over 5 to 10 generations. In response to foraging, one prey type becomes poisonous, one prey type becomes semi-poisonous, and one prey type remains palatable. To simulate the cost of defense, prey reproduction rate is dependent on level of prey defense. Co-evolution and predator specialization are simulated by allowing one predator type to adapt to the poisonous prey species.

16. Energetic Strategies of Terrestrial Vertebrates
Kathy Winnett-Murray and Lori H. Hertel (Hope College)

A major objective of this lab is to link organismal biology and ecology through the exploration of vertebrate metabolic strategies and their ecological consequences. Two components are completed in the 3-hour lab. First, students investigate the difference in metabolic response of representative endotherms (mice) and ectotherms (green anoles) to temperature changes. Metabolic rate is gauged via oxygen consumption using an oxygen analyzer. In the second component, ecological consequences are studied through behavior and preferred body temperature in a temperature gradient using simple gradient boxes and electronic thermometers.

Friday, June 4
All workshops presented 8:00 am - 11:00 am and 1:30 pm -4:30 pm unless noted otherwise

17. What I Could Teach Darwin Using "Darwin 2000", an Interactive Web Site for Student Research into the Evolution of Genes and Proteins
Rick Hershberger (Carlow College)

The "Darwin 2000" web site (www.bioactivesite.com/biocomputing/darwin2000/) was developed to train undergraduates in the use of online molecular sequence databases and analysis tools, and to guide their research into the evolution of genes and proteins. Workshop participants will (a) locate DNA and protein sequences of their choice within GenBank, (b) search for homologous genes or proteins, (c) conduct a multiple sequence alignment to identify conserved regions within protein families and examine molecular phylogenies, and (d) map conserved domains on molecular models. All databases, biocomputing servers, and modeling software used are available free online. Instructional technology and pedagogical considerations in designing the site will also be discussed.

18. Introduction to the Molecular Phylogeny of Insects
Theodore Gurney, Robert Elbel, Dhitinut Ratnapradipa (University of Utah), and Robert Bossard (Washington State University)

Characterization of insect species by PCR will be illustrated with four lab exercises: (1) Rapid DNA isolation from single insects such as fleas, ants, fruit flies, or mosquitoes. The procedure can also be used with many other eukaryotic organisms. Students may bring their own specimens (preserved in 95% ethanol) or may use ours. (2) PCR using universal ITS primers. We will supply previously isolated insect DNA. (3) Electrophoresis of PCR products. We will supply PCR products. (4) Calculations of PCR product sizes. We will also provide information for further characterization of insect DNA by RFLP and by sequencing.

19. Shifts in Microhabitat Use by Snails in Response to Fish Predators
Timothy W. Stewart and Charlene Waggoner (Bowling Green State University)

Natural selection promotes evolution of predator detection and avoidance strategies, especially in species lacking physical defenses. Using a laboratory experiment, we will illustrate mechanisms of predator detection and avoidance in thin-shelled pond snails (Physella). Specifically, we will test hypotheses that snails 1) detect predators through chemical cues, and 2) alter microhabitat use (i.e., leave the water or increase use of physical structure) after detecting predators. A Mann-Whitney test will be used to quantify predator-mediated changes in snail microhabitat use. Finally, we will introduce a follow-up experiment that evaluates the efficacy of microhabitat shifts in reducing fish effects on snail mortality.

20. Courtship Reaction Chains and Signals of Melittobia Parasitic Wasps
Judith A. Guinan and Robert W. Matthews (University of Georgia-Athens)

Insect courtship is very diverse, with some species exhibiting complex and highly stereotyped courtship sequences. In this lab, students will (1) document the sequence of events (referred to as reaction chains) that comprise the courtship of a common species of parasitic wasp, Melittobia digitata, and (2) examine the effect of mating experience on the timing and duration of courtship. They will then design and perform a simple bioassay experiment to determine whether pheromones play particular roles in mate attraction. Compared to Drosophila courtship laboratories, Melittobia are easily manipulated, require no anesthesia, and the sexes are clearly distinct.

21. Evolution By Artificial Selection and Unraveling the Mysteries of Hairy's Inheritance
Daniel Lauffer (University of Wisconsin- Madison)

Using rapid-cycling Brassica rapa, Wisconsin Fast Plants, a nine-week exercise has been developed for an introductory biology course in artificial selection. This tested activity results in substantial evolution over two consecutive generations for the number of trichomes present on the petiole of the first true leaf. Selecting for either an increased or decreased population value, students compare their experimental results with their predictions for mean and distribution. Results are used to predict the effects of continued selection over subsequent generations. The intermating of the two divergent populations, hairy and hairless, leads to further investigations into quantitative inheritance.

22. Investigations of Aquatic Food Web Interactions Using Microcosms
Kyle D. Hoagland and John C. Holz (University of Nebraska-Lincoln)

Plankton assemblages from lakes and reservoirs offer significant opportunities to investigate both basic and applied ecological principles at the community level, including fundamental processes such as competition, herbivory, and predation. This exercise will make use of microcosms to examine a variety of ecological interactions characteristic of aquatic food webs by selective additions of phosphorus and/or fish, thus focusing on trophic cascades and testing top-down versus bottom-up hypotheses. It incorporates student use of quantitative skills to enumerate zooplankton and phytoplankton, data collation, and statistical analysis, as well as the concepts of sound experimental design, adequate replication, and inference. Indirect interactions within communities are also evident using this approach.

23. Individualized Laboratory Instruction: Problems and Solutions in the Project-Based Laboratory
John Tidswell (University of New Brunswick)

Individualized project-based learning allows students to explore their own interests within a structured context, simulates the real-world situation, and places educational responsibility on the student. Through presentation, discussion and planning exercises, participants will explore the costs and benefits of this instructional method and investigate problems and practical solutions. This workshop will be presented only 1:30 - 4:30 pm.



Mini Workshops

Between the morning and afternoon sessions of the major workshops a number of half-hour and hour mini workshops will be presented. Sign-up sheets for these workshops will be available at the registration desk. A sampling of the titles of the mini workshops to be presented are listed below:

  • Zebrafish Development: A Module for Introductory Biology Laboratories Combining Direct Observation, Experimental Manipulations, and WEB-based Resources
  • Physiological Ecology Lab Exercise: Phenotypic Plasticity in Sun and Shade Leaves
  • Labs Don't Have to be Wet
  • Antigen-Antibody Interactions: Application of the Ouchterlony Test for Determining Evolutionary Relationships
  • Using Simulation Software to Create Laboratory Experiences in Lecture Courses
  • Death by Osmosis
  • The Botany Field Site (WWW): A Herbarium Project and A guide through the Northeastern Deciduous Forest

Special Events

Welcome Reception

A welcome reception and buffet, sponsored by the College of Arts and Sciences and the School of Biological Sciences at the University of Nebraska-Lincoln, will be held on Tuesday evening, June 1 from 6:30 - 8:30 pm in Elephant Hall at the University of Nebraska State Museum. Meet new ABLE members and rekindle old acquaintances as you walk among the world famous collection of elephant skeletons and their close fossil relatives. Many of the fossil remains of mammoths, mastodons, and four tuskers on display in Elephant Hall were unearthed in Nebraska, including the world's largest exhibited elephant skeleton, a 14 foot male "Imperial Mammoth" from the Late Pleistocene of Lincoln County, Nebraska. A life-sized bronze statue of this mammoth (nicknamed Archie) was recently installed at the main entrance to the Museum. During the evening you will have the opportunity to take a leisure, self-guided tour of the museum exhibits which interpret the natural, geological and anthropological history of Nebraska and the Great Plains. The reception is free of charge to all conference participants.

Special Lecture

Date: Wednesday, June 2, 11:30 - 12:30, 115 Burnett Hall

Agricultural Biotechnology - Some lessons from my research on the study of plant viruses. T. Jack Morris, Director and Distinguished Professor, School of Biological Sciences, University of Nebraska-Lincoln.

Dr. Morris is a plant pathologist/biologist who studies the molecular biology of small RNA plant viruses. His lab has pioneered studies on the molecular biology of small RNA plant viruses of the Tombusvirus and Carmovirus groups, some of the best characterized of any RNA viruses infecting eukaryotes. The research is focused on the understanding fundamental aspects of virus assembly, RNA replication and recombination, and the molecular basis of virus-host interactions in plant systems including Arabidopsis and tomato. His virology expertise has been redirected recently as part of UNL's team of researchers focused on the genetic engineering of plants. The group is interested in exploring novel ways to genetically engineer plants with disease resistance and to use plants for the production of foreign proteins such as vaccines directed against human and animal viruses.

Pow Wow
     Date: Wednesday evening, June 2
     Cost: $20
Dances and the ceremonies with which they are associated have always been a very important part of the life of all Native Americans, no matter what their tribe. Dances, or "Pow Wow's" as they are now called, are occasions for renewing old acquaintances, making new friends, and when held on reservations, for reaffirming tribal ties. The Pow Wow will feature an evening of dancing and singing by members of several Southern Plains tribes. All tribal dances are preceded by a feast for which the Great Spirit's blessing is asked. In keeping with this tradition, a light meal of Southern Plains Indian food will be served. Shuttle buses from the Pound Residence Hall will transport conference participants to the Lincoln Indian Center, about 3 miles from campus, where the Pow Wow will take place.

Conference Dinner - A Tropical Experience!
     Date: Friday evening, June 4
     Cost: $35
The conference dinner will be held in the Lied Jungle complex at the Henry Doorly Zoo in Omaha, Nebraska. The Lied Jungle is the world's second largest indoor tropical rainforest. Visitors experience the sights, sounds and smells of the jungle as they meander through recreations of Asian, African and South American rain forests. A path along the jungle floor winds along streams and past waterfalls which pour 750,000 gallons of water per hour into the jungle. An elevated walkway takes visitors through treetops and offers a glimpse of nearly 3000 species of plants and 125 species of animals including free ranging reptiles, birds and primates as well as other mammals. Dr. Lee Simmons, Director of the Henry Doorly Zoo, our host for the evening, will give participants a behind the scenes tour of the Jungle. This will be followed by a buffet dinner in the Tree Tops Restaurant which overlooks the Jungle. Buses will leave from the Pound Residence Hall for the one hour trip to Omaha, which is located 60 miles east of Lincoln.



Pre-Conference Field Trips (#1 - 3)

Tuesday, June 1

ABLE conference field trips are meant to be educational experiences for the adult attendees. Individuals age 17 or older who are accompanied by a conference participant will be allowed to attend field trips if space is available and will be expected to pay the full field trip fee. All pre-conference field trips will leave from the parking lot adjacent to the Pound Residence Hall.

Field Trip 1: University of Nebraska Agricultural Research & Development Center - $25.

Agriculture is Nebraska's primary industry. There are more than 56,000 farms and ranches ranging from small-scale vegetable farms to several-thousand-acre cattle operations. Beef cattle, hogs, corn, soybeans, wheat, and popcorn are primary to the industry. As a result, UNL supports a great deal of agriculture related research and development. On this field trip participants will visit one of the University of Nebraska's Agricultural Research and Development Centers (ARDC). This facility is composed of 20 units including Ag Meteorology, Conservation and Survey, Cow/Calf, Dairy, Veterinary and Biomedical Sciences, Sheep, Swine and Horticulture/Turf. The faculty and staff at the ARDC will give participants a behind-the-scenes tour of the various units and explain the research being conducted in each unit. Some highlights will include: a tour of the largest irrigated turf research facility in the U.S., a tour of the 1200 capacity cattle feedlot, a demonstration of precision farming technologies using GIS/GPS, herds of cattle and sheep used in studies of reproductive physiology and endocrinology, field crop production research including both irrigated and dryland crops, a livestock waste composting project which composts about 3 tons of manure annually, and a tour of the "shower-in-shower out" swine facility. The ARDC staff have promised participants an all-you-can-eat lunch and an afternoon snack of ice cream and pop. Wear an old pair of walking shoes which you won't mind getting contaminated with a bit of "livestock waste"!

Field Trip 2: Exploring the Natural and Human History of the Big Bend region of the Platte River - $25.

The Platte River originates near North Platte, Nebraska. where the North and South Platte rivers join. From there, it flows eastward along a 310 mile S-shaped course and ultimately empties into the Missouri River near Omaha. Ecologically it is one of the most important rivers in the United States. Plant and animal communities from across the nation meet and co-exist along the Platte River. The Big Bend region of the Platte in central Nebraska, is a critically important stretch of the river. Each year, more than 10 million ducks, geese and shorebirds, three-quarters of the world's population of sandhill cranes, and Whopping Cranes use this area to feed and rest during spring migration. Staff from The Nature Conservancy and the Crane Trust will lead participants on a field trip to explore the biodiversity of the river and surrounding floodplain and outline the efforts being undertaken to protect and manage the river's resources and surrounding habitats. After eating a box lunch, we will take a short tour of the Stuhr Museum of the Prairie Pioneer in Grand Island. The museum features exhibits which reflect the contrasting cultures of the plains Indians and the early western settlers who migrated through the Platte River Valley following he Great Platte River Road - the combination of the Oregon, Mormon, and California Trails which carried and estimated 360,000 settlers west in years between 1841 and 1870. The adjacent 40 acre living history Railroad Town is patterned after those along the Union Pacific right-of-way in the 1880's. The field trip cost includes a box lunch and the admission fee to the museum. Bring sun screen, insect repellent, and wear a pair of shoes that can get wet.

Field Trip 3: Planes, Fish, and Wildlife - $25.

The first stop on this trip will be the new Strategic Air Command (SAC) Museum, 30 miles east of Lincoln. The museum features a world-class collection of aircraft, missiles and other aerospace artifacts as well as a restoration unit where historic planes are prepared for display. More than 30 historic aircraft, including the legendary B-36 "Peacemaker" and the awesome SR71 "Blackbird" are on display in two immense hangars. The second stop on the field trip will be the Ak-Sar-Ben Aquarium Outdoor Education Center. This facility houses 12 aquaria, the largest holding 1450 gallons of water, which display fish native to Nebraska or which have been successfully introduced. We will eat lunch at the park and participants can take a leisurely walk along the nature trails. The third and last stop on the field trip will be at the 7000 acre DeSoto National Wildlife Refuge which is located along the Missouri River, 25 miles north of Omaha. The refuge hosts a large variety of waterfowl, shorebirds, song birds, and birds of prey. In addition, the refuge houses a fascinating archeological exhibit of artifacts excavated from the sunken remains of the steamboat Bertrand which sank in 1865, near the present site of the refuge. The field trip cost includes a box lunch and admission fees. Wear walking shoes and bring sun screen and insect repellent.



Post Conference Field Trips (# 4 - 6)

Saturday, June 5

ABLE conference field trips are meant to be educational experiences for the adult attendees. Individuals age 17 or older who are accompanied by a conference participant will be allowed to attend field trips if space is available and will be expected to pay the full field trip fee. All post-conference field trips will leave from the parking lot adjacent to the Pound Residence Hall.

Field Trip 4: Ashfall Fossil Beds State Historical Park - $40.

The Ashfall fossil beds are situated on 360 acres of rugged rangeland in the scenic Verdigre Creek Valley near Royal, Nebraska. Ten million years ago a massive volcano erupted in the Rocky Mountains, producing a cloud of volcanic ash that suffocated and buried rhinos, three-toed horses, and camels around a watering hole. Whole herds of animals died and were buried so quickly that their carcasses are largely intact. Some of the best preserved fossil rhinos, horses, camels, birds, and turtles known anywhere have been, and are being, excavated in the Ashfall Fossil Beds. The Ashfall site has been featured on the NBC Nightly News, a PBS special titled "Mysteries in the Dust", and in National Geographic magazine and the National Geographic book, Giants From the Past. Dr. Mike Voorhies, Curator of Vertebrate Paleontology at the University of Nebraska State Museum, will be our host at Ashfall. Mike was responsible for making the first significant discoveries at the site in 1971 and has been actively working the site since then. Travel to and from Ashfall will be by motorcoach. We will stop at the Neligh Mill Historic Site for a casual box lunch along the Elkhorn River and a brief tour of the Neligh Mill, a circa 1874 flour mill. At the end of the day, participants will be treated to a meal of down-home Nebraska cooking at the Green Gables of Pleasant Valley Restaurant. The field trip cost includes a box lunch, dinner, and admission fees. It is suggested that you wear walking shoes and bring along sun screen and a water bottle.

Field Trip 5: Konza Prairie - $40.

The Konza Prairie Research Natural Area, located just outside Manhattan, Kansas, is the largest remaining tract of tall grass prairie in North America. The 3487 hectare (8600 acre) tract of tall grass prairie is managed by Kansas State University. Konza is part of the NSF Long Term Ecological Research (LTER) program. Major research projects focus on the affects of fire and grazing on tall grass prairies. A managed herd of 200 bison serve as native grazers in research projects. Data collected at Konza on the role of bison on the structure of tall grass prairie was featured in the December 1998 issue of BioScience. Transportation to and from Konza will be by van. We will make a rest stop in Marysville, KS for a snack and a short tour of the Pony Express Station Museum, the only original home station along the Pony Express Route that still stands in its original location. Box lunches will be provided on site at the prairie. Before departing Manhattan we will stop for dinner at the Little Apple Brewing Company which features typical mid-western cuisine and fine micro-brewed beer. The field trip cost includes a box lunch, dinner, and the admission fees. Participants should wear comfortable walking shoes and bring along sun screen and a water bottle.

Field Trip 6: Indian Cave State Park and Squaw Creek National Wildlife Refuge - $30.

This field trip will feature the natural history and geology of the Missouri River bluffs and floodplain in southeast Nebraska and northwest Missouri. You will travel by van through vast expanses of crop land to Squaw Creek National Wildlife Refuge near Mound City, Missouri. This 7,350 acre refuge is home for 31 kinds of mammals, 35 species of reptiles and amphibians, and 301 species of birds. Migratory birds stop to rest and feed at Squaw Creek Refuge during their spring and fall migration. The refuge includes areas of loess bluff hills, an unusual geologic formation caused by wind deposited soil. Some of the last remnants of the native prairie are found on these bluffs. Refuge roads and foot trails provide access to many wildlife observation areas. After a box lunch break at the refuge visitors center, we will return to Nebraska and spend a leisurely afternoon exploring Indian Cave State Park which spans a 3052-acre area along the Missouri River. The park is named for a natural cave created by silt and fine-grained sand deposits in a Pennsylvanian rock channel. Petroglyphs or ancient Indian picture writings etched on the walls of the cave are the only known example of their kind found in Nebraska. This is a fascinating area with scenic vistas, hundreds of birds and other wildlife, and many varieties of plants that are unique in Nebraska. The park also features the reconstructed mid-19th Century river settlement known as St. Deroin which was originally established as a settlement for the homeless offspring left behind by traders and trappers who married Indian women but later served as a trading and supply post for river traffic. At the end of the day, field trip participants will be treated to a cookout featuring buffalo burgers (barbecued tofu for the vegetarians), corn bread, salad, and beverage. Participants should wear a good pair of walking shoes or hiking boots and bring along insect repellent and sun screen



Visit Nebraska

The organizing committee extends an invitation to conference participants to come early and stay late to explore Nebraska's diverse biological habitats and historic sites. For example, the Sandhills occupy a major portion of the western part of the state. These dunes are the largest grass-stabilized sand dunes in the world and overlie the Ogallala aquifer which bubbles to the surface forming thousands of small lakes and wetland areas. For more information about the Sandhills and other areas of interest to visit while in Nebraska, check out the following web sites: http://visitnebraska.org/ and http://ngp.ngpc.state.ne.us/

 


All contents copyright © 2005. Association for Biology Laboratory Education. All rights reserved.