Title: Detecting Genetic Polymorphisms in Different Populations of Bean Beetle (Callosobruchus maculatus)
Url: http://www.ableweb.org/volumes/vol-35/v35reprint.php?ch=17
Author: Karen Hicks
Jennifer McMahon-Smith
Author's Address: Kenyon College, Department of Biology, 202 N. College Rd., Gambier OH 43022 USA
Email Address: smithje@kenyon.edu
Description: Differences at the DNA sequence level are intrinsically important in categorizing groups of organisms. This exercise introduces students to DNA extraction and PCR, with which they will compare different populations of bean beetles (Callosobruchus maculatus). Using primer sets generated from the Callosobruchus genome, students compare DNA amplification patterns via electrophoresis. DNA regions selected for amplification have been identified as containing microsatellites, which often contain numerous polymorphisms useful in assessing genetic similarities. Students are asked to consider how separation of populations may affect changes at the DNA level. This exercise offers participation in new research, and contributes to bean beetle biology.
Keywords: PCR
bean beetle
STR
Callosobruchus
microsatellites
Topic: Genetics
Evolution
Molecular Biology
BEN Subject/Discipline Taxonomy: Molecular Biology
Genetics & Heredity
Evolutionary Biology
Learning Resource Type: Laboratory exercise (DCMI Type Vocabulary)
Context: Undergraduate lower division 13-14
Undergraduate upper division 15-16
Format: pdf
Access Rights: This resource is open access.
Rights: http://www.ableweb.org/copyright-policy/
Conference Location & Year: University of Calgary (2013)
Cumulative Rating: NOT YET RATED
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