Title: Pests Be Gone! Or Not?! Looking at the Effect of an Organophosphate Insecticide on Acetylcholinesterase Activity in the Bean Beetle
Url: http://www.ableweb.org/volumes/vol-35/v35reprint.php?ch=52
Author: Fardad Firooznia
Hector Fermin
Gurcharan Singh
Min Gyu Noh
Violeta Contreras Ramirez
Rahat Gul
Author's Address: City College of New York, Department of Biology, MR-526,160 Convent Ave. New York, NY 10031 USA
Email Address: hector@sci.ccny.cuny.edu
Description: Here we describe an enzyme assay to look at the effect of the organophosphate insecticide malaoxon on the activity of acetylcholinesterase (AChE) in the bean beetle Callosobruchus maculatus. Malaoxon inhibits the activity of AChE and interferes with neuronal activity. The procedure involves a crude protein extraction and a colorimetric assay to determine enzyme activity. The procedure is also used to investigate whether the inhibition by the insecticide is competitive or non-competitive. These lab exercises tie in several topics together: data processing and presentation, enzymatic reactions and enzyme inhibitors, cell-cell signaling, and biological applications to industry and their potential ecological consequences.
Keywords: enzyme assay
Callosobruchus
bean beetle
acetylcholinesterase
Topic: Animal Physiology
Cell Biology
Biochemistry
BEN Subject/Discipline Taxonomy: Entomology
Physiology
Cell biology
Biochemistry
Enzymology
Agriculture & Aquaculture
Learning Resource Type: Laboratory exercise (DCMI Type Vocabulary)
Context: Undergraduate lower division 13-14
Undergraduate upper division 15-16
Format: pdf
Access Rights: This resource is open access.
Rights: http://www.ableweb.org/copyright-policy/
Conference Location & Year: University of Calgary (2013)
Cumulative Rating: NOT YET RATED
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