Gene expression is an essential concept to biology majors. In this two-week guided inquiry laboratory,
students are led to discover the role of promoters in gene expression using colorimetric assays measurable
by spectrophotometry. Additionally, the two-week lab module aims to introduce students to elements of
research including iterative skills, data analysis and data interpretation by comparison of their data to
assigned figures in primary research papers. Students use a straight-forward protocol to perform the betagalactosidase
assay to analyze four Saccharomyces cerevisiae strains carrying the lacZ reporter transgene
driven by a eukaryotic wild-type or mutant promoter.