Description:
This year we introduced a hands-on lab activity that presented second year undergraduates with
the opportunity to practice the fundamental techniques of growing and maintaining mammalian
cell lines. We were able to achieve this in a class of 300 students by dividing the students into
small groups which rotated between three stations. Students were introduced to sterile technique in
the lab and did a mock run through the procedure, and then moved to a tissue culture room to
subculture HEK 293 cells. Finally, students proceeded to a third station to count cells and
determine cell viability using the Trypan Blue exclusion test. This practical lab activity was well
received and despite significant organizational demands in advance, ended up running smoothly
and efficiently. During this workshop I will describe our process of implementing this activity,
and will demonstrate our approach to teaching sterile techniques used in tissue culture. As a group
we will consider benefits and limitations of this type of lab, and will discuss alternate ways of
incorporating practical cell culture techniques into large classes. |
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| Learning Resource Type: |
Laboratory exercise (DCMI Type Vocabulary)
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| Access Rights: |
This resource is for ABLE members only. |
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