Title: Quick, Safe and Simple Silver Staining for Ciliates and Other Protists
Url: http://www.ableweb.org/volumes/vol-37/v37reprint.php?ch=63
Author: Taylor Newman
Ben Waggoner
Author's Address: University of Central Arkansas, Department of Biology, AR
Email Address: ediacara@cyberback.com
Description: In ciliated protists (Ciliophora), the basal bodies (kinetids) are arranged in species-specific patterns that are maintained from one generation to the next. Kinetids stain strongly when exposed to silver compounds. So does the ciliate "silverline system," the edges of the alveolar sacs underneath the plasma membrane. Most techniques for silver-staining ciliates are technically difficult and require toxic reagents. We present a simple, quick, inexpensive, and safe stain technique that reveals the complexity of ciliates and some other protists. Our procedure can easily be used in a project-based unit on protist diversity, or in inquiry-driven labs for secondary and post-secondary students.
Keywords: staining
Protista
basal bodies
ciliates
silver
Topic: Microbiology
Teaching Tools and Techniques
Cell Biology
BEN Subject/Discipline Taxonomy: Biodiversity
Microbiology
Structural biology
Invertebrate Biology
Cell biology
Teaching Tools and Techniques
Learning Resource Type: Laboratory exercise (DCMI Type Vocabulary)
Context: Undergraduate lower division 13-14
Undergraduate upper division 15-16
Format: pdf
Access Rights: This resource is for ABLE members only.
Rights: http://www.ableweb.org/copyright-policy/
Conference Location & Year: Boston University (2015)
Cumulative Rating: NOT YET RATED
Resource Comments:

(no comments available yet for this resource)

Log In:


Create a Login Create a Login
Forget your password? Forget your password?