Title: Microsatellite DNA: Population Genetics and Forensic Applications
Url: http://www.ableweb.org/volumes/vol-33/v33reprint.php?ch=2
Author: Kuei-Chiu Chen
Author's Address: Cornell University, Neurobiology and Behavior, NY
Email Address: kc373@cornell.edu
Description: Microsatellite DNA has been used as standard genetic markers for human identification in forensic laboratories. This multi-week lab module uses professional lab techniques to cover concepts in molecular biology and mathematics. Students who have given consent extract DNA from their cheek cells and conduct a multiplex polymerase chain reaction that amplifies ten microsatellite loci simultaneously. The PCR products are analyzed using a standard DNA analyzer and then further processed with Peak Scanner (free software). A full genetic profile can achieve a random match probability as low as one in a trillion. The analysis of a genetic profile can be further expanded to parentage determination.
Keywords: population genetics
Hardy-Weinburg genetic equilibrium
molecular marker
forensics
polymerase chain reaction
Topic: Genetics
Molecular Biology
BEN Subject/Discipline Taxonomy: Genetics & Heredity
Molecular Biology
Population Biology
Learning Resource Type: Laboratory exercise (DCMI Type Vocabulary)
Context: Undergraduate lower division 13-14
Format: pdf
Access Rights: This resource is for ABLE members only.
Rights: http://www.ableweb.org/volumes/copyright.htm
Conference Location & Year: New Mexico State University (2011)
Cumulative Rating: NOT YET RATED
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