Tested Studies for Laboratory Teaching
Volume 21

University of Nebraska-Lincoln, June 1-5, 1999

Editor: Susan J. Karcher (Purdue University)
Chair of Host Committee: Bill Glider


1. What I Could Teach Darwin Using "Darwin 2000", an Interactive Web Site for Student Research into the Evolution of Genes and Proteins by Richard P. Hershberger (Division of Natural Sciences and Mathematics, Carlow College) [abstract] [full text]

2. Case It! - case-study learning integrating molecular biology computer simulations and international Internet conferencing by Mark Bergland, Karen Klyczek, Mary Lundeberg, Kim Mogen, and Douglas Johnson (Department of Biology, University of Wisconsin-River Falls) [abstract] [full text]

3. Introduction to the Molecular Phylogeny of Insects by Theodore Gurney, Robert Elbel, Dhitinut Ratnapradipa, and Robert Bossard (Department of Biology, University of Utah) [abstract] [full text]

4. Seafood Forensics: Crabby Proteins by Kathy Frame (National Association of Biology Teachers) [abstract] [full text]

5. Introducing Students to Conservation Genetics Using Sturgeon Caviar by Kathleen A. Nolan, Tony Catalano, Phaedra Doukakis, Vadim Birstein, and Rob DeSalle (American Museum of Natural History) [abstract] [full text]

6. Population Genetics and Evolution: A Simulation Exercise by Christine K. Barton (Division of Science and Mathematics, Centre College) [abstract] [full text]

7. Investigating an Immune Response to Bacterial Infection by Jon S. Miller (Department of Biological Sciences, Northern Illionois University) and David W. Stanley (Department of Entomology, University of Nebraska-Lincoln) [abstract] [full text]

8. Evolution By Artificial Selection and Unraveling the Mysteries of Hairy's Inheritance by Daniel Lauffer (Department of Plant Pathology, University of Wisconsin-Madison) and Bruce Fall (General Biology Program, College of Biological Sciences and University of Minnesota) [abstract] [full text]

9. An Introduction to Plant Vascular Systems by Laura K. Thompson (Biology Department, Furman University) [abstract] [full text]

10. Induction of Nitrate Reductase in Plant Shoots by Barry G. McCashin (Department of Biological Sciences, University of Alberta) [abstract] [full text]

11. Effects of Irradiance on Photosynthetic CO2 Uptake and Chlorophyll Fluorescence by Stephen Hunt (Department of Biology, Queen's University) [abstract] [full text]

12. Electromyography: Recording Electrical Signals from Human Muscle by Charlie Drewes (Department of Zoology and Genetics, Iowa State University) [abstract] [full text]

13. Microhabitat Shifts By Snails In Response To Fish Predators by Timothy W. Stewart (Department of Natural Sciences, Longwood College) and Charlene M. Waggoner (Department of Biological Sciences, Bowling Green State University) [abstract] [full text]

14. Insect Predation, Prey Defense, and Community Structure by W. Wyatt Hoback (Department of Biology, University of Nebraska at Kearney) and Leon G. Higley (Department of Entomology, University of Nebraska-Lincoln) [abstract] [full text]

15. Aquatic Food Web Interactions: Microcosms as Lake Models by John C. Holz (School of Natural Resource Sciences and University of Nebraska), Kyle D. Hoagland (School of Natural Resource Sciences and University of Nebraska), and Anthony Joern (School of Biological Sciences and University of Nebraska) [abstract] [full text]

16. Paleoecology as a Classroom Tool to Address Global Climate Change by Mark E. Lyford (Department of Botany, University of Wyoming) and Jane M. Beiswenger (Department of Botany and Department of Zoology and Physiology, University of Wyoming) [abstract] [full text]

17. Quantitative investigation of the crowding effect of Hymenolepis diminuta in Rattus norvegicus by Sara V. Brant and Ben Hanelt (Department of Biological Sciences, University of Nebraska) [abstract] [full text]

18. Energetic Strategies of Terrestrial Vertebrates by Kathy Winnett-Murray, K. Greg Murray, Lori Hertel, and Christopher C. Barney (Department of Biological Sciences, Hope College) [abstract] [full text]

19. Courtship Reaction Chains and Mate Attraction: A Two-Part Laboratory Activity Using WOWBugs, A New Model Insect by Judith A. Guinan (Institute of Ecology), Robert W. Matthews, and Janice R. Matthews (Entomology Department, University of Georgia) [abstract] [full text]

20. An Experimental System to Study Phagocytosis by Donna M. Bozzone and Denise A. Martin (Department of Biology, Saint Micheal's College) [abstract] [full text]

21. Creating Interactive Biology Tutorials for the World Wide Web Using a Simple Java Editor by William Udell (Center for Instructional Innovation, University of Nebraska-Lincoln) [abstract] [full text]

22. Effective Methods of Training Biology Laboratory Teaching Assistants by Maggie Haag , Kimberley Christopher (Department of Biological Sciences, University of Alberta), John Cummings, Jean Dickey (Department of Biology Instruction and Agricultural Education, Clemson University), and Bill Glider (School of Biological Sciences, University of Nebraska-Lincoln) [abstract] [full text]

Appendix A: Abstracts of Mini Workshops  [titles]


Abstracts(Vol. 21)

1 -- What I Could Teach Darwin Using "Darwin 2000", an Interactive Web Site for Student Research into the Evolution of Genes and Proteins
Richard P. Hershberger [full text]
Key words: bioinformatics, sequence databases, sequence analysis, molecular modeling, GenBank, homology, molecular phylogenetics, hemoglobins, blast.
The "Darwin 2000" website (http://www.bioactivesite.com/biocomputing/darwin2000/) was developed to train undergraduates to use online molecular sequence databases and analysis tools, and to foster their understanding of how genes and proteins evolve. Students conduct a research project by (a) locating DNA and protein sequences of their choice within GenBank, (b) searching for homologous genes or proteins using BLAST, (c) conducting multiple sequence  alignments to identify conserved regions within protein families and examine molecular phylogenies, and (d) mapping conserved domains on molecular models. The databases, biocomputing servers, modeling software, and Darwin 2000 web-based instructional materials are available free online.

2 -- Case It! - case-study learning integrating molecular biology computer simulations and international Internet conferencing
Mark Bergland, Karen Klyczek, Mary Lundeberg, Kim Mogen, and Douglas Johnson [full text]
Key Words: case, Internet, conferencing, simulation, molecular, disease, DNA, electrophoresis.
Case It! is an NSF-supported project initiated by participants in the BioQUEST Curriculum Consortium. The overall goal is to develop a framework for collaborative case-based learning in molecular biology using interactive computer simulations, and to have students from around the world participate in web-based "poster sessions" via Internet conferencing. Software modules described in this chapter can be downloaded from the Case It! web site at no cost to educators (http://www.uwrf.edu/caseit/caseit.html). Results of class-testing are also available at this web site. Contact mark.s.bergland@uwrf.edu for additional information concerning the project, and to learn how you and your students can participate.

3 -- Introduction to the Molecular Phylogeny of Insects
Theodore Gurney, Robert Elbel, Dhitinut Ratnapradipa, and Robert Bossard [full text]
Key Words: insects, systematics, evolution, DNA, polymerase chain reaction, intervening transcribed sequences, ribosomal RNA.
This lab introduces DNA analysis as a tool for the study of phylogeny.  Using the same PCR primers different insect species can have different sizes of PCR products which can be resolved by simple agarose gel electrophoresis. The lab consists of four exercises, 1) DNA isolation from single insects, 2) PCR using universal ITS primers, 3) Electrophoresis of PCR products, and 4) Calculation of the sizes of the PCR products. Sizes of the PCR products from unknown insects can be compared with the sizes from known insect species.

4 -- Seafood Forensics: Crabby Proteins
Kathy Frame [full text]
Key Words: Protein analysis, horizontal gel electrophoresis, agarose, high molecular weight proteins.
Students perform protein analysis of crab tissue to determine if species substitution has occurred. Samples are analyzed using horizontal agarose gel electrophoresis. This hands-on, investigative, "cutting-edge" activity is one of 17 in the publication Shoestring Biotechnology. The publication emphasizes biotechnology's role in the environment, medicine, plant and animal conservation, and agriculture, as well as how to teach biotech cost-effectively (NSF/ATE/DUE #9553720).

5 -- Introducing Students to Conservation Genetics Using Sturgeon Caviar
Kathleen A. Nolan, Tony Catalano, Phaedra Doukakis, Vadim Birstein, and Rob DeSalle [full text]
Key Words: sturgeon, caviar, conservation, forensics, polymerase chain reaction, species specific primers.
In this laboratory exercise students will learn how to: (a.) Isolate DNA from individual sturgeon eggs (available at any local deli that sells caviar) using the Quanta-Genomic method, (b.) Set up control and species-specific PCR reactions using primers that have been developed for DNA from sturgeon species and (c.) Employ electrophoresis and methylene blue and/or ethidium bromide staining to visualize the PCR products. This laboratory exercise allows students to contribute to a growing DNA database on endangered species.

6 -- Population Genetics and Evolution: A Simulation Exercise
Christine K. Barton [full text]
Key words: population genetics; evolutionary simulation; Hardy-Weinberg, gene flow, genetic drift, natural selection.
The Hardy-Weinberg law represents the cornerstone of the modern theory of evolution. In an infinitely large population with completely random mating, this law establishes a mathematical model that allows us to predict accurately both gene and genotype frequencies. Biologists use the predictions of the Hardy-Weinberg law as a standard against which microevolutionary change can be measured. In this lab exercise, students are presented with a hands-on simulation using colored plastic beads that allows them to create an equilibrium population. By manipulating this initial population, students then simulate the subsequent effect that (a) gene flow, (b) natural selection, and (c) genetic drift could have on the original gene pool. The hands-on simulation is supplemented with a computer exercise.

7 -- Investigating an Immune Response to Bacterial Infection
Jon S. Miller and David W. Stanley [full text]
Key words: cellular immunity, hemocytes, nodulation, Manduca sexta.
The ability of organisms to defend themselves from parasites and pathogens is a fundamental aspect of biology. Because of the risk of infection from blood-born pathogens, and the elaborate protocols required to handle and keep mammals and other vertebrate organisms, it is becoming more difficult and expensive to conduct laboratory exercises that illustrate fundamental immune reactions. Alternative models are needed to teach this important biological process. Insects are excellent models of cellular immune reactions to bacterial infections. We present activities that are appropriate for today's high school, college, and university biology teaching laboratories. Using larvae of the tobacco hornworm, Manduca sexta, students gain a basic understanding of techniques employed to identify various hemocyte types involved in cellular immune reactions, become familiar with the normal appearance of various internal structures, and conduct an assessment of nodule formation in response to a bacterial infection.  Laboratory Exercises in Plant Biology

8 -- Evolution By Artificial Selection and Unraveling the Mysteries of Hairy's Inheritance
Daniel Lauffer and Bruce Fall [full text]
Key Words: Wisconsin Fast Plants, artificial selection, evolution, quantitative inheritance.
Using rapid-cycling Brassica rapa, Wisconsin Fast Plants, a nine-week exercise for a large introductory liberal education biology course that involves the students as participants in artificial selection has been developed. This tested activity results in substantial evolution over two consecutive generations for the number of trichomes present on the petiole of the first true leaf. Selecting for either an increased or decreased population value, students compare their experimental results with their predictions for mean and distribution. Results are used to predict the effects of continued selection over subsequent generations. The intermating of the two divergent populations, hairy and hairless, leads to further investigations into quantitative inheritance.

9 -- An Introduction to Plant Vascular Systems
Laura K. Thompson [full text]
Key words: plant vascular systems, tissue sectioning, xylem transport, stomata.
One of the most important features of plants which make them suited to life on dry land is the vascular system which distributes water, nutrients and sugars throughout the plant from their disparate sources (leaves, roots, atmosphere, soil, etc.). This freshman level laboratory exercise, which introduces students to plant diversity, uses an investigative approach to study various aspects of the plant vascular system. Students study the anatomy of the xylem system in a dicot stem, measure the xylem flow rate, and study the effect of microenvironmental conditions that control stomatal opening.

10 -- Induction of Nitrate Reductase in Plant Shoots
Barry G. McCashin [full text]
Key words: nitrate reductase, enzyme induction, corn shoots.
The induction of enzyme activity by a substrate is usually examined in prokaryotes but in this lab, we investigate the phenomenon in a higher plant. Using a simple colorimetric assay, we study the induction of nitrate reductase in the shoots of corn seedlings after applying a solution containing nitrate to the roots. We look at the effect of nitrate concentration as well as the time course of enzyme induction. We also assess the role of light in the process.

11 -- Effects of Irradiance on Photosynthetic CO2 Uptake and Chlorophyll Fluorescence
Stephen Hunt [full text]
Key Words: Photosynthesis, Chlorophyll Fluorescence, CO2 Fixation, Irradiance, Quantum Yield.
This investigation introduces students to the concept of measuring photosynthesis by open flow gas analysis using a CO2 analyzer to measure CO2 uptake by a leaf in a leaf chamber. At the same time chlorophyll fluorescence is also measured. The photon flux supplied to the leaf is altered and effects on photosynthetic rate and chlorophyll fluorescence are measured. Students plot the relationships between irradiance, photosynthetic rate, quantum yield and photosynthetic electron transport rate. The investigation provides background for numerous other studies into the effects of environmental perturbations on photosynthetic physiology.  Laboratory Exercises in Physiology

12 -- Electromyography: Recording Electrical Signals from Human Muscle
Charlie Drewes [full text]
Key words: motor unit, EMG, biofeedback, movement, reflexes.
Students will use surface recording electrodes, a small battery-powered pre-amplifier, and a small battery-powered audio-monitor (for purposes of biofeedback) to analyze electromyogram (EMG) activity from the gastrocnemius muscle in a freely moving, untethered human. Also, students (1) learn principles of EMG recording, (2) study electrical activity patterns from single motor units, (3) examine patterns of motor unit recruitment during voluntary movement, (4) study the graded nature of involuntary stretch reflex responses, (5) measure stretch reflex latency with an oscilloscope, and (6) calculate average sensori-motor conduction velocity in human leg nerve.  Laboratory Exercises in Ecology, Evolution, and Behavior

13 -- Microhabitat Shifts By Snails In Response To Fish Predators
Timothy W. Stewart and Charlene M. Waggoner [full text]
Key words: antipredator behavior, chemical cues, predator-prey interactions, physical structural complexity, Physella, snails, gastropods, redear sunfish.
Natural selection promotes evolution of predator-detection and avoidance strategies, especially in species lacking physical defenses. In this exercise, an experiment is used to illustrate mechanisms of predator detection and avoidance in thin-shelled pond snails (Physella).  Specifically, investigators test hypotheses that snails 1) detect predators through chemical cues, and 2) alter microhabitat use (i.e., leave the water or increase use of physical structure) after detecting predators.  Paired-sample t tests are used to quantify predator-mediated changes in snail microhabitat use. Finally, as an extension to this exercise, we introduce an experiment that evaluates efficacy of physical structure in reducing fish effects on snail mortality.  

14 -- Insect Predation, Prey Defense, and Community Structure
W. Wyatt Hoback, and Leon G. Higley [full text]
Key Words: Predation, Prey Defense, Ecology, Insects.
Insect predators use one of three general feeding strategies with associated morphologies (mandibles, raptorial forelegs, and unmodified legs) to capture prey. Evolutionarily, species subjected to strong predation pressure may respond by developing defenses (including poison) or by producing more offspring. This exercise examines the interplay between three potential prey species and three insect predator types played by the students. Simulations are conducted over 5 to 10 generations with changes in population size being tracked. In response to foraging, one prey type becomes poisonous, one prey type becomes semi-poisonous, and one prey type remains palatable. To simulate the cost of defense, prey reproduction rate is dependent on the level of prey defense. Co-evolution and predator specialization are simulated by allowing one predator type to adapt to poisonous prey species.

15 -- Aquatic Food Web Interactions: Microcosms as Lake Models
John C. Holz, Kyle D. Hoagland, and Anthony Joern [full text]
Key Words: aquatic food webs, trophic interactions, phosphorus, phytoplankton, zooplankton, planktivorus fish, indirect interactions, factorial design.
Increasing knowledge of trophic cascades in aquatic ecosystems has led to a better understanding of several basic ecological tenets, including competition, predation, and a variety of other community-level food web interactions. We have developed a simple exercise using microcosms to demonstrate these ecological interactions for use in limnology, aquatic ecology, or general ecology courses. This lab exercise incorporates student use of rigorous quantitative skills in enumeration, data collection, and statistical inference. Whole water plankton collections are added to standard 15-gallon aquaria, allowed to equilibrate, then manipulated by adding planktivorous fish and/or phosphorus, with the appropriate controls. Algae and zooplankton are sampled periodically throughout the 7-10 day experiment, and/or on the final day of the study. Differences in species composition and biomass of the dominant taxa are then compared to determine the effects of fish, nutrients, and their combination on community structure. Indirect interactions are also evident using this approach. Thus, this exercise provides students with experience with standard techniques of data collection and analysis in limnology, concepts in community and population ecology, an introduction to a specific application of the scientific method, and  insights into contemporary research approaches in aquatic ecology.

16 -- Paleoecology as a Classroom Tool to Address Global Climate Change
Mark E. Lyford and Jane M. Beiswenger [full text]
Key Words: paleoecology, climate change, Pleistocene, pollen.
We have designed an activity to illustrate paleoecological techniques with a focus on the Pleistocene period. We provide students with background information and ask them to count pollen on slides representing six different time periods from a site in Idaho. Based on the class data, we ask the students to interpret how the vegetation has changed at the site.  The students then infer how the climate would have changed given the ecological characteristics of the species examined. We conclude with a discussion of how interpreting past vegetation changes may help predict future vegetation patterns in response to climate change.

17 -- Quantitative investigation of the crowding effect of Hymenolepis diminuta in Rattus norvegicus
Sara V. Brant and Ben Hanelt [full text]
Key Words: tapeworm, cestode, Hymenolepis, crowding effect, Rattus, parasites.
Parasitism is the prevailing life-style and host-parasite associations provide outstanding models in studies of species  interactions. This exercise demonstrates some basic principles in parasitology using a simple and easily obtainable system. The following will be presented: (1) An introduction to the relationship of Hymenolepis diminuta with its rodent host; (2) An overview of methods used in experimental studies; (3) Extrapolation of results to the impacts on the host and other  individual parasites. Through experimentation, the students will understand what is a parasite, details of a complex life cycle, and how parasites interact with their environment.

18 -- Energetic Strategies of Terrestrial Vertebrates
Kathy Winnett-Murray, K. Greg Murray, Lori Hertel, and Christopher C. Barney [full text]
Key Words: vertebrate energetics, endotherm, ectotherm, Anolis carolinensis, Mus musculus.
A major objective of this lab is to link organismal biology and ecology through the exploration of vertebrate metabolic strategies and their ecological consequences. Two components are completed in the 3-hr lab.  First, students investigate the difference in metabolic response of representative endotherms (mice) and ectotherms (green anoles) to temperature changes. Metabolic rate is gauged via oxygen consumption using an oxygen analyzer. In the second experiment, ecological consequences are studied through behavior and preferred body temperature in a temperature gradient using simple gradient boxes and electronic thermometers.  

19 -- Courtship Reaction Chains and Mate Attraction: A Two-Part Laboratory Activity Using WOWBugs, A New Model Insect
Judith A. Guinan, Robert W. Matthews, and Janice R. Matthews [full text]
Key Words: mating, ethogram, Hymenoptera, behavior sampling methods, bioassay, sex pheromone.
Insect courtship is diverse, often including complex, highly stereotyped behavioral sequences. As an alternative to Drosophila fruit flies for studies of insect courtship, this activity introduces Melittobia digitata, commonly called WOWBugs. Melittobia have a rapid life cycle, produce large numbers of easily manipulated adults, and require no anesthesia. In the first exercise, students observe Melittobia courtship; describe its components; document their sequence and duration; and develop a courtship reaction chain. The second exercise is a directed inquiry using a simple choice chamber to investigate whether WOWBugs produce a signal for mate attraction, and if so, what form the signal takes.

20 -- An Experimental System to Study Phagocytosis
Donna M. Bozzone and Denise A. Martin [full text]
Key words: Tetrahymena, cells, phagocytosis, vacuole, cytoskeleton, cilia, nutrition.
We present a simple technique to observe and quantitate phagocytosis in the protozoan, Tetrahymena. The procedure consists of feeding ink to cells and counting the number of vacuoles that form over a 30-minute time period.  Because the protocol is straightforward and the cell behavior is interesting to observe, this system is excellent both for introducing students to the microscope and for inquiry-based laboratory investigations.  Information is presented explaining how to set up experiments to observe phagocytosis and to examine factors affecting this process. Specific parameters considered include the effects of 1) ink concentration, 2) the cell's nutritional state, and 3) cytoskeletal inhibitors. We also consider possibilities for further experimentation.  Instructional Materials

21 -- Creating Interactive Biology Tutorials for the World Wide Web Using a Simple Java Editor
William Udell [full text]
Key Words: interactive tutorials, WEB, Jamba, animations.
This hands-on workshop provided participants an opportunity to work with Jamba , an easy to use and inexpensive Java editor from Interleaf Software. This program can be used to create interactive tutorials for the Internet including: drag and drop, animation, fill in the blank and multiple-choice questions, and interactive slide shows. Workshop participants constructed a basic drag and drop concept map and then created an interactive animation dealing with water balance in living cells. This tutorial uses a mastery based learning strategy in which the student is allowed to move on to the next question only after having successfully answered the previous question. The Jamba software is currently available for Windows 95 and Windows NT platforms only. However, tutorials created with this software will run on Windows 95, Windows NT, Unix, and Macintosh platforms.  Information about Jamba can be found on the World Wide Web at http://www.jamba.com.

22 -- Effective Methods of Training Biology Laboratory Teaching Assistants
Maggie Haag, Kimberley Christopher, John Cummings, Jean Dickey, and Bill Glider [full text]
Key Words: TA training programs, manuals, videotapes, Classroom dilemmas.
One of the most important factors in the success of undergraduate biology laboratories rests with the instructors that teach them. Often, especially in multi-section courses, this responsibility is left in the hands of graduate or undergraduate teaching assistants (TAs). In recent years many universities and colleges have begun to put an effort into TA training, focusing on effective and often varied means of instruction, clear and concise methods of communication, and consistent and fair marking. Some of this training is carried out by campus-wide teaching and learning centers while other aspects of this training is provided at the departmental level. This workshop presented a number of examples of programs used for the training of TAs teaching biology (Clemson University, University of Alberta, University of Nebraska-Lincoln) as well as some methodologies that have proven successful.


Mini Workshops

  • How To Solve Genetics "Word Problems" by Kathy Wiley Schwab (Biology Department, Huston-Tillotson College) [full text]
  • The Red and White Yeast Lab: An Introduction to Science As A Process by Brian White (Biology Department, University of Massachusetts Boston) [full text]
  • Antigen-Antibody Interaction: using the Ouchterlony method to support taxonomic relationships by Mary Culp (Department of Biology, Canisius College) [full text]
  • Death by Osmosis: An interactive, investigative laboratory exercise by William M. Beachly (Department of Biology, Hastings College) [full text]
  • Teaching Buffering by Comparing Observed and Expected Hydrogen and Hydroxide Ion Change by Robert J. Kosinski and John R. Cummings (Department of Biology Instruction and Agricultural Education, Clemson University) [full text]
  • A Simulated Pollination Exercise by R. Paul Willing (Biology Department, Union College) [full text]
  • The Botany Field Site (WWW): An Herbarium Project and A Guide through the Northeastern Deciduous Forest by Jacqueline McLaughlin (Biology Department, Pennsylvania State University) [full text]
  • Grafting Coleus Plants by Karin Readel (Interdisciplinary Science Program, University of Maryland) [full text]
  • Physiological Ecology Lab Exercise: Phenotypic Plasticity in Sun and Shade Leaves by Jill Goldstein (Institute of Ecology, University of Georgia), Robert Wyatt (Highlands Biological Station), and Jim Richardson (Institute of Ecology, University of Georgia) [full text]
  • Group Projects for the Botany Lab by Debora L. Mann (Biology Department, Millsaps College) [full text]
  • Models and Mindsets for Teaching Neurophysiology by Charlie Drewes (Zoology and Genetics Department, Iowa State University) [full text]
  • Labs Don't Have To Be Wet by Roberta Williams (Biology Department, University of Nevada) [full text]
  • Using a Mini-Symposium to Display the Results of Team-Designed Experiments to Encourage Freshman Pride & Teamwork by Denise Marie Ratterman (Department of Biology, Saint Joseph's University) [full text]
  • The Use of Writing in Investigative Biology Laboratories by Ralph W. Preszler (Department of Biology, New Mexico State University) [full text]
  • Using Simulation Software to Create Laboratory Experiences in Lecture Courses by Ruth E. Beattie (T.H. Morgan School of Biological Sciences, University of Kentucky) [full text]


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