Detecting Genetic Polymorphisms in Different Populations of Bean Beetle (Callosobruchus maculatus)

 

Tested Studies in Laboratory Teaching, 2014, Volume 35

Jennifer McMahon Smith & Karen A. Hicks

Abstract

Differences at the DNA sequence level are intrinsically important in categorizing groups of organisms. This exercise introduces students to DNA extraction and PCR, with which they will compare different populations of bean beetles (Callosobruchus maculatus). Using primer sets generated from the Callosobruchus genome, students compare DNA amplification patterns via electrophoresis. DNA regions selected for amplification have been identified as containing microsatellites, which often contain numerous polymorphisms useful in assessing genetic similarities. Students are asked to consider how separation of populations may affect changes at the DNA level. This exercise offers participation in new research, and contributes to bean beetle biology.

Keywords:  PCR, Callosobruchus, bean beetle, microsatellites, STR

University of Calgary (2013)