Characterization of Worm Capture by Nematophagous Fungi: Real Research in the Classroom

 

Tested Studies in Laboratory Teaching, 2015, Volume 36

Brian Sato

Abstract

Discovery driven experiments in undergraduate laboratory courses have been shown to increase student learning and critical thinking abilities. This workshop focuses on a lab module involving the nematophagous fungus, Arthrobotrys oligospora, an ecologically relevant organism with potential use as a pest control agent in agricultural settings, and its ability to capture the nematode Caenorhabditis elegans. The goals of this module are to enhance scientific understanding of the regulation of worm capture by soil dwelling fungi and for students to attain a set of established learning goals. Groups of four students are provided with the experimental background and conduct their own literature search to identify a variable that may affect the efficiency of C. elegans capture. Students develop a hypothesis and conduct an experiment to compare worm survival in the control versus variable condition, writing a lab report in the format of a primary research article. From this experimental module, students were able to produce results that agree with published data as well as add to the existing literature, while demonstrating positive gains regarding the learning objectives. Workshop attendees will learn about nematophagous fungi, design potential experiments that their students could perform using the established module, and get hands on experience with the organisms and experimental protocol. In addition, we will discuss how to incorporate this module into your lab curriculum along with potential means of assessment to measure student learning.

Keywords:  nematode, predatory fungi

University of Oregon (2014)