Identification of T Lymphocytes

 

Tested Studies in Laboratory Teaching, 1997, Volume 18

Abstract

In a clinical setting, it is often necessary to examine the cells of the immune system, especially in patients with immune deficiencies. One method takes advantage of the fact that lymphocytes will bind foreign red blood cells to form rosettes which can be enumerated microscopically. Specifically, the Ox49/50 antigen on the rat T lymphocyte surface has been identified as the probable binding site of the sheep rbcs. In this exercise, we will show that the number of antigen-binding lymphocytes increases after immunization.

Keywords:  immunization, lymphocyte, cell-mediated immunity, rosette

Boston University (1996)