The Hill Reaction: In Vitro and In Vivo Studies

 

Tested Studies in Laboratory Teaching, 1994, Volume 15

Abstract

For many students, light-driven electron-transport can be abstract and with little utility. To provide additional ways of visualizing this process, this series of exercises is designed (1) to show that the light-reactions of photosynthesis are proportional to light intensity and can be monitored in isolated chloroplast-particles, (2) to demonstrate that chlorophyll, in solution, traps and re-emits light by fluorescence, and (3) to observe increases in chlorophyll fluorescence in intact green-algae when cultures are treated with herbicides that block electron transport. These exercises were adapted from Laboratory Exercises in Plant Physiology by D. E. Balint and E. A. Funkhouser (Ginn Press, 1993).

Keywords:  green algae, photosynthesis, light reaction, herbicide

University of Toronto (1993)