Tested Studies in Laboratory Teaching, 1995, Volume 16
Abstract
Monoclonal antitubulin antibodies produced in mice will be used to label bull sperm microtubules and the location of these antibodies will be determined by use of a peroxidase conjugated secondary antibody and the DAB staining reaction. Protocols are presented for use in a second-year student laboratory class given in a multi-section (repeated) format. These protocols include those for preparation of poly-L-lysine coated slides and of bull sperm, as well as those for immunocytochemistry, using the DAB reaction.
Keywords: sperm, cytoskeleton, immunocytochemistry, DAB staining
Emory University (1994)
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