Introducing Students to Conservation Genetics Using Sturgeon Caviar and Other Fish Eggs

 

Tested Studies in Laboratory Teaching, 2004, Volume 25

Abstract

In this laboratory exercise students will learn how to: (a) Isolate DNA from individual sturgeon and other fish eggs (available at any local deli that sells caviar) using the DNAzol method, (b) Set up control and species-specific PCR reactions using primers that have been developed for DNA from sturgeon species and (c) Use electrophoresis and methylene blue and/or ethidium bromide staining to visualize the PCR products. This laboratory exercise would allow students to contribute to a growing DNA database on endangered species.

Keywords:  PCR, forensics, polymerase chain reaction, caviar, sturgeon, conservation genetics, species-specific primers

University of Nevada, Las Vegas (2003)