While many e-cigarette users perceive vaping as a healthier alternative to smoking, there is evidence that the
effects of e-cigarettes are not necessarily healthier than regular cigarettes (Reidel et al. 2018). Even a single
exposure to e-cigs has been shown to cause excessive inflammation and mucus production that could lead to
airway obstruction (Gellatly et al. 2020). Secondhand nicotine vape exposure has also been associated with an
increased risk of bronchitis symptoms and shortness of breath among young adults (Islam et al. 2022). While
students may read about all this research or be informed via a lecture, the purpose of implementing four hands-
on activities is to allow students various models of scientific exploration so that they may conduct actual or
simulated research for themselves as well as interpret unbiased graphical data to make their own informed
conclusions regarding the negative health impacts of vaping. The activities include two activities (graphical
analysis and simulated biomarkers) from a research data analysis project created by UNC?s Center for Public
Engagement with Science, of which the activities are also free on their website. A hands-on lab that allows
participants to look directly at the effects of e-cigarette vapor on living cells: the protozoan Tetrahymena, a
model organism often used in research on health and disease-related topics, including ciliary function, from
Washington University in St. Louis. A novel activity utilizes a small closed system in which non-nicotine vape
juice is emitted through a micro fog machine. Students will analyze changes in air quality using air monitoring
devices. The four activities have been utilized by BioNetwork staff independently of each other and in various
combinations of two or more activities.
Keywords: vaping, health risks, biomarkers, environmental monitoring, model simulations, data interpretation, hands-on, graphical analysis, EVALI
University of Maryland (2024)
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