One challenge for students in a biochemistry lab course can be the lack of visual cues in their experiments;
students may perceive labs as exercises in mixing small volumes of clear liquids. We have developed a fusion
protein of mCherry-GUS that is an ideal biochemistry lab tool. This fusion protein is robustly expressed, easily
purified, exceptionally stable, brightly colored under ambient light, and capable of performing economical
colorimetric assays. Students can intuitively track this fusion protein and monitor enzymatic reactions by
following the colored protein and colorimetric reactions in each experiment. mCherry-GUS can be used across
many weeks to teach expression, purification, enzymatic activity and kinetics; and this workshop will consist of
learning those experiments. Further, these experiments can be done with either micro or transfer pipettes ?
making them accessible to budget conscious labs and students from high school through undergraduate.
Keywords: biochemistry, enzymes, protein purification, colorimetric assay
University of Maryland (2024)
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