Association for Biology Laboratory Education

Identify unknown blue-green algae with traditional techniques and DNA sequence data
    

Christopher A. Sheil, Michael P Martin, Nathan Yaussy, and Jeffrey R Johansen

Advances in Biology Laboratory Education, 2025, Volume 45

https://doi.org/10.37590/able.v45.art19

Abstract

Students are often intrigued by biodiversity. However, students can be intimidated by survey courses that require memorizing synapomorphies with little context, and associated survey labs often provide few opportunities to develop skills used to identify unknown organisms. Herein, students receive ?unknown? blue-green algae, and practice skills to identify/name new species. This laboratory can be run with algae collected locally, or it can be a collaboration on a CURE with John Carroll University faculty. Students refine their statements of sample identity while developing four skills: Skill 1, illustrate an unknown specimen of cyanobacteria; Skill 2, use a dichotomous key to determine the family and genus to which the unknown likely belongs; Skill 3, perform a BLAST search on GenBank to determine the genus and species that their unknown sample most closely resembles; and Skill 4, examine the placement of unknown taxa on a phylogeny to make inferences about taxonomy and classification.

Keywords:  CURE, Cyanophyta, NCBI, taxonomy, phylogeny

University of Maryland (2024)