Association for Biology Laboratory Education

Using computational tools to visualize SARS-CoV-2 genome variation
    

April E. Williams and Rachael M. Barry

Advances in Biology Laboratory Education, 2025, Volume 45

https://doi.org/10.37590/able.v45.abs38

Supplemental Materials: https://doi.org/10.37590/able.v45.sup38

Abstract

Computational and quantitative skills are important for a well-rounded modern biology education. In this workshop, we present a standalone lesson created for undergraduate microbiology courses that provides an introduction to data visualization in R using the Posit Cloud interface. In this lesson, students follow a step-by- step protocol to analyze sequencing data of SARS-CoV-2 genome variants using pre-made R scripts. They create a map of mutations found across the genome as well as a series of graphs depicting the types of mutations observed and their frequency. A series of questions throughout the activity promote group discussion about topics in genetics and virology. These questions also encourage students to make predictions about the data based on their prior knowledge and compare them to the maps and graphs they create, building authentic research skills. The final part of the lesson requires students to make modifications to the provided R code in order to display data about a gene of their choosing within the virus genome. This lesson requires no prior coding or R experience. All data analysis is done using free cloud computing resources and requires no specialized equipment other than computer and internet access.

Keywords:  SARS-CoV-2, COVID-19, genome, mutation, R, genetics, microbiology, virology

University of Maryland (2024)