Members of the Pennsylvania Academy of Science collaborated to develop a manual that pulls together several
research-based labs that they have previously run in their upper-division college lab courses. The activities
include bacteria, yeast, and fruit fly microbiology, zebrafish-based cell biology, invertebrate and plant
metagenomics, bioinformatics, and genetic engineering in bacteria and yeast. Advanced laboratory techniques
include end-point PCR, qPCR, RT-qPCR, bar coding, in situ hybridization, in silico and in vitro gene cloning and
editing, and site-directed mutagenesis and mutation detection. The activities can be combined to create multi-
week lab explorations or run individually as standalone experiments.
Keywords:
University of Maryland (2024)
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