Gene knockout/gene therapy in yeast using homologous recombination

 

Tested Studies in Laboratory Teaching, 2009, Volume 30

Ginny G. Hutchins & S. Catherine Silver Key

Abstract

This laboratory module has been implemented at several undergraduate institutions in North Carolina and Colorado in genetics and molecular biology courses above the introductory level. The basis of this module is the same technology used to create targeted gene deletions in S. cerevisiae yeast (baker's yeast) in research laboratories, either for individual genes or systematically for the entire yeast genome. Coupling the engaging concept of ‘gene therapy' with the complex concept of ‘homologous recombination', this laboratory learning module is designed to facilitate student learning of abstract concepts and their application to real world problems.

Keywords:  recombination, yeast, knockout

University of Toronto, Mississauga (2008)