Regulation of meiosis in S. cerevisiae

 

Tested Studies in Laboratory Teaching, 2010, Volume 31

Skokotas, A & E. Winter

Abstract

This laboratory exercise was created for general biology or sophomore level genetics students. It can be used as a supplement to a mitosis/meiosis lab that typically examines prepared slides of the cell cycle. This exercise allows for the examination of live mitotic and meiotic cells. The budding yeast Saccharomyces cerevisiae is an outstanding system to study these processes. In this exercise, we have developed an assay for trapping cells at the meiotic commitment point (Prophase I) in order to study the effect of glucose in meiotic regulation.

Keywords:  meiosis, mitosis, cell cycle in yeast, yeast genetics

University of Delaware (2009)