Genotyping SNPs Associated with Dyslexia

 

Tested Studies in Laboratory Teaching, 2011, Volume 32

Ann Yezerski

Abstract

This exercise is designed to teach every aspect of a genotyping project by using a Single Nucleotide Polymorphism (SNP) in the KIAA0319 gene that is known to be associated with dyslexia. Initially, students extract their own DNA and then set-up subsequent PCR reactions and restriction digests in order to determine their genotype. Concurrent exercises in Bioinformatics demonstrate how a genotyping protocol is designed, as well as how phenotype can vary due to a single base pair change. As a result, this exercise demonstrates the multitude techniques that a research scientist uses to investigate how SNPs contribute to human phenotypes.

Keywords:  PCR, bioinformatics, SNP, proteomics, genotyping, dyslexia

Dalhousie University (2010)