Tested Studies in Laboratory Teaching, 1995, Volume 16
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Abstract
Monoclonal antitubulin antibodies produced in mice will be used to label bull sperm microtubules and the location of these antibodies will be determined by use of a peroxidase conjugated secondary antibody and the DAB staining reaction. Protocols are presented for use in a second-year student laboratory class given in a multi-section (repeated) format. These protocols include those for preparation of poly-L-lysine coated slides and of bull sperm, as well as those for immunocytochemistry, using the DAB reaction.
Keywords: sperm, cytoskeleton, immunocytochemistry, DAB staining
Emory University (1994)
aAs of September 1, 2026
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