A study of fermentation by Saccharomyces cerevisiae

 

Janice M. Bonner

Tested Studies in Laboratory Teaching, 2009, Volume 30

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Abstract

This workshop, derived from an article in American Biology Teacher (Reinking, Reinking, and Miller, 1994) presents a simple method for investigating fermentation. In the basic reaction, a 7% yeast suspension is combined with a 1.0% glucose solution in fermentation tubes—conical centrifuge tubes in which the caps have small perforations. As CO2 is produced, the yeast-sugar solution leaves through the hole. At regular intervals, students mark off the fluid level in the fermentation tubes and measure the concentration of glucose with a diagnostic test strip. A small amount of data manipulation is necessary to obtain the volume of CO2 produced by fermentation. The basic reaction can be carried out within 15 minutes.

Keywords:  enzyme, yeast, fermentation

University of Toronto, Mississauga (2008)

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